GenepHlow PCR Cleanup Kit

The GenepHlow™ PCR Cleanup Kit was designed to recover or concentrate DNA fragments from PCR or other enzymatic reactions. This PCR cleanup kit includes a pH indicator as an optional addition to the PB binding buffer to ensure optimal pH and facilitate DNA binding. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.

Advantages:

• High Efficiency: up to 95% recovery from PCR or other enzymatic reactions
• Only 10 minute PCR cleanup
• Includes a pH Indicator for easy determination of optimal pH
• Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
• Sample Size: up to 100 μl of PCR products
• Broad Fragment Size Range: 70 bp–20 kb
• Gel/PCR glass fiber spin columns (blue membrane optimized for PCR cleanup)
• Storage: dry at room temperature (15-25°C) for up to 1 year